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What is a Bronchial Provocation Test (Methacholine Challenge Test)?
A Bronchial Provocation Test, also known as a Methacholine Challenge Test, is a specialized lung function test used to help diagnose asthma and airway hyperresponsiveness. This test evaluates how sensitive your airways are to certain triggers by exposing them to methacholine, a substance that may cause mild narrowing of the airways in people with reactive or sensitive lungs.
Why is a Methacholine Challenge Test Done?
This test is commonly recommended for individuals who experience:
- Chronic cough
- Wheezing
- Shortness of breath
- Other asthma-like symptoms
especially when results from standard pulmonary function tests (PFTs), such as spirometry, appear normal. The Methacholine Challenge Test helps confirm or rule out asthma in cases where other tests are inconclusive.
How the Bronchial Provocation Test Works?
During the test:
You inhale small, controlled doses of methacholine through a nebulizer.
After each dose, your breathing is measured using a spirometer to record your FEV₁ (Forced Expiratory Volume in one second).
If your lung function drops by 20% or more, this indicates airway hyperreactivity, which is a key feature of asthma.
A bronchodilator is administered at the end of the test to reverse any airway constriction.
Is the Methacholine Challenge Test Safe?
Yes, the test is considered safe when performed under the supervision of trained healthcare professionals. It is not typically used for individuals who already have a clear asthma diagnosis or severely reduced lung function.
Key Takeaways:
- The Methacholine Challenge Test is a reliable tool for diagnosing asthma when other tests are normal.
- It helps detect airway sensitivity and reactivity.
- The procedure is safe, quick, and often covered by insurance with a doctor’s referral.
Other PFT Tests
Why Choose a Bronchial Provocation Test?

Highly Sensitive for Detecting Asthma
Airway reactivity even if symptoms are mild

Assesses Airway Hyperrespon-siveness
Helps diagnose exercise-induced or occupational asthma

Quick & Non-Invasive
A simple breathing test performed under medical supervision

Safe & Controlled Procedure
The test is carefully monitored, and medications can quickly reverse any effects
What Does a Bronchial Provocation Test Detect?

Asthma & Airway Sensitivity
Confirms if your airways overreact to triggers

Exercise-Induced Asthma
Helps assess breathing issues related to physical activity

Chronic Cough Causes
Determines if asthma is behind a persistent cough

Airway Disorders
Identifies breathing issues that mimic asthma

Occupational Asthma
Diagnoses asthma caused by workplace irritants
Want to Consult a Doctor Before Your Bronchial Provocation Test?
If you’re unsure whether this test is necessary, consult a pulmonologist for expert advice.
Why Consult a Doctor?
✔️ Confirm whether your symptoms indicate asthma or another lung condition
✔️ Get expert advice on managing airway sensitivity
✔️ Understand if additional tests like Spirometry or DLCO Test are needed
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How to Prepare for a ABG Test?
- Avoid caffeine & smoking for at least 24 hours before the test
- Do not take asthma medications unless instructed by your doctor
- Wear comfortable clothing for easy breathing
- Inform your doctor if you have any heart conditions or severe breathing issues
- Follow medical guidance before undergoing the test
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